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monoclonal antigapdh 60004 1 ig antibody  (Proteintech)
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Proteintech monoclonal antigapdh 60004 1 ig antibody
Monoclonal Antigapdh 60004 1 Ig Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antigapdh 60004 1 ig antibody - by Bioz Stars, 2026-03
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60004 1 ig  (Proteintech)
96
Proteintech 60004 1 ig
60004 1 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/60004 1 ig/product/Proteintech
Average 96 stars, based on 1 article reviews
60004 1 ig - by Bioz Stars, 2026-03
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anti gapdh  (Proteintech)
99
Proteintech anti gapdh
Anti Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gapdh/product/Proteintech
Average 99 stars, based on 1 article reviews
anti gapdh - by Bioz Stars, 2026-03
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gapdh  (Proteintech)
99
Proteintech gapdh
Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdh/product/Proteintech
Average 99 stars, based on 1 article reviews
gapdh - by Bioz Stars, 2026-03
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mouse anti gapdh  (Proteintech)
99
Proteintech mouse anti gapdh
Human ataxin-3 proteolysis is calpain dependent. A, The effect of calpain or caspase inhibition on the presence of ataxin-3 cleavage fragments at 3 dpf was examined by immunoblotting protein lysates from control or EGFP-ataxin-3-84Q larva following 2 d of treatment with DMSO, calpain inhibitor compounds (ALLN or calpeptin), a pan-caspase inhibitor (zVAD-fmk), or a cathepsin inhibitor (CAT-1). The immunoblot was probed with an antibody against <t>cleaved</t> <t>spectrin,</t> ataxin-3, and a loading control (β-actin or <t>GAPDH).</t> The first lane of each immunoblot contained control samples (nontransgenic) and the subsequent lanes contained EGFP-ataxin-3-84Q samples treated with DMSO and increasing concentrations of the protease inhibitor. Calpeptin (50, 100, 200 μm) treatment produced a dose-dependent increase in the amount of full-length ataxin-3 and decrease in ataxin-3 cleavage product. Exposure to ALLN produced a similar result to calpeptin treatment. Treatment with zVAD-fmk or CAT-1 failed to preserve full-length ataxin-3 or decrease the amount of cleavage fragments. B, Quantification of levels of full-length ataxin-3 within separate immunoblots (n = 3–6) for treatment with each drug revealed that 25–50 μm calpeptin significantly increased the amount of full-length ataxin-3 compared with DMSO treatment, *p < 0.04. C, Quantification of the level of ataxin-3 cleavage fragments within samples of zebrafish treated with each protease inhibitor compound revealed a trend of decreased level of cleavage fragments following treatment with calpain inhibitor compounds, with 100 μm calpeptin significantly decreasing levels of the cleavage fragment (p = 0.031). FL, Full-length; CF, cleavage fragment; ZF, zebrafish. Error bars represent mean ± SEM.
Mouse Anti Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti gapdh/product/Proteintech
Average 99 stars, based on 1 article reviews
mouse anti gapdh - by Bioz Stars, 2026-03
99/100 stars
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mouse antigapdh antibody  (Proteintech)
99
Proteintech mouse antigapdh antibody
Human ataxin-3 proteolysis is calpain dependent. A, The effect of calpain or caspase inhibition on the presence of ataxin-3 cleavage fragments at 3 dpf was examined by immunoblotting protein lysates from control or EGFP-ataxin-3-84Q larva following 2 d of treatment with DMSO, calpain inhibitor compounds (ALLN or calpeptin), a pan-caspase inhibitor (zVAD-fmk), or a cathepsin inhibitor (CAT-1). The immunoblot was probed with an antibody against <t>cleaved</t> <t>spectrin,</t> ataxin-3, and a loading control (β-actin or <t>GAPDH).</t> The first lane of each immunoblot contained control samples (nontransgenic) and the subsequent lanes contained EGFP-ataxin-3-84Q samples treated with DMSO and increasing concentrations of the protease inhibitor. Calpeptin (50, 100, 200 μm) treatment produced a dose-dependent increase in the amount of full-length ataxin-3 and decrease in ataxin-3 cleavage product. Exposure to ALLN produced a similar result to calpeptin treatment. Treatment with zVAD-fmk or CAT-1 failed to preserve full-length ataxin-3 or decrease the amount of cleavage fragments. B, Quantification of levels of full-length ataxin-3 within separate immunoblots (n = 3–6) for treatment with each drug revealed that 25–50 μm calpeptin significantly increased the amount of full-length ataxin-3 compared with DMSO treatment, *p < 0.04. C, Quantification of the level of ataxin-3 cleavage fragments within samples of zebrafish treated with each protease inhibitor compound revealed a trend of decreased level of cleavage fragments following treatment with calpain inhibitor compounds, with 100 μm calpeptin significantly decreasing levels of the cleavage fragment (p = 0.031). FL, Full-length; CF, cleavage fragment; ZF, zebrafish. Error bars represent mean ± SEM.
Mouse Antigapdh Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse antigapdh antibody/product/Proteintech
Average 99 stars, based on 1 article reviews
mouse antigapdh antibody - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier

Image Search Results


Human ataxin-3 proteolysis is calpain dependent. A, The effect of calpain or caspase inhibition on the presence of ataxin-3 cleavage fragments at 3 dpf was examined by immunoblotting protein lysates from control or EGFP-ataxin-3-84Q larva following 2 d of treatment with DMSO, calpain inhibitor compounds (ALLN or calpeptin), a pan-caspase inhibitor (zVAD-fmk), or a cathepsin inhibitor (CAT-1). The immunoblot was probed with an antibody against cleaved spectrin, ataxin-3, and a loading control (β-actin or GAPDH). The first lane of each immunoblot contained control samples (nontransgenic) and the subsequent lanes contained EGFP-ataxin-3-84Q samples treated with DMSO and increasing concentrations of the protease inhibitor. Calpeptin (50, 100, 200 μm) treatment produced a dose-dependent increase in the amount of full-length ataxin-3 and decrease in ataxin-3 cleavage product. Exposure to ALLN produced a similar result to calpeptin treatment. Treatment with zVAD-fmk or CAT-1 failed to preserve full-length ataxin-3 or decrease the amount of cleavage fragments. B, Quantification of levels of full-length ataxin-3 within separate immunoblots (n = 3–6) for treatment with each drug revealed that 25–50 μm calpeptin significantly increased the amount of full-length ataxin-3 compared with DMSO treatment, *p < 0.04. C, Quantification of the level of ataxin-3 cleavage fragments within samples of zebrafish treated with each protease inhibitor compound revealed a trend of decreased level of cleavage fragments following treatment with calpain inhibitor compounds, with 100 μm calpeptin significantly decreasing levels of the cleavage fragment (p = 0.031). FL, Full-length; CF, cleavage fragment; ZF, zebrafish. Error bars represent mean ± SEM.

Journal: The Journal of Neuroscience

Article Title: Calpain Inhibition Is Protective in Machado–Joseph Disease Zebrafish Due to Induction of Autophagy

doi: 10.1523/JNEUROSCI.1142-17.2017

Figure Lengend Snippet: Human ataxin-3 proteolysis is calpain dependent. A, The effect of calpain or caspase inhibition on the presence of ataxin-3 cleavage fragments at 3 dpf was examined by immunoblotting protein lysates from control or EGFP-ataxin-3-84Q larva following 2 d of treatment with DMSO, calpain inhibitor compounds (ALLN or calpeptin), a pan-caspase inhibitor (zVAD-fmk), or a cathepsin inhibitor (CAT-1). The immunoblot was probed with an antibody against cleaved spectrin, ataxin-3, and a loading control (β-actin or GAPDH). The first lane of each immunoblot contained control samples (nontransgenic) and the subsequent lanes contained EGFP-ataxin-3-84Q samples treated with DMSO and increasing concentrations of the protease inhibitor. Calpeptin (50, 100, 200 μm) treatment produced a dose-dependent increase in the amount of full-length ataxin-3 and decrease in ataxin-3 cleavage product. Exposure to ALLN produced a similar result to calpeptin treatment. Treatment with zVAD-fmk or CAT-1 failed to preserve full-length ataxin-3 or decrease the amount of cleavage fragments. B, Quantification of levels of full-length ataxin-3 within separate immunoblots (n = 3–6) for treatment with each drug revealed that 25–50 μm calpeptin significantly increased the amount of full-length ataxin-3 compared with DMSO treatment, *p < 0.04. C, Quantification of the level of ataxin-3 cleavage fragments within samples of zebrafish treated with each protease inhibitor compound revealed a trend of decreased level of cleavage fragments following treatment with calpain inhibitor compounds, with 100 μm calpeptin significantly decreasing levels of the cleavage fragment (p = 0.031). FL, Full-length; CF, cleavage fragment; ZF, zebrafish. Error bars represent mean ± SEM.

Article Snippet: Antibodies used included rabbit anti-MJD (kind gift from H. Paulson), rabbit anti-beclin-1 (11306-1-AP, Proteintech), rabbit anti-p62 (SQSTM1, MBL PM045, MBL Life Science), rabbit anti-LC3B (ab51520, Abcam), mouse anti-spectrin (sc-48382, Santa Cruz Biotechnology), mouse anti-β-actin (A5441, Sigma-Aldrich) and mouse anti-GAPDH (60004-1, Proteintech) for loading controls.

Techniques: Inhibition, Western Blot, Protease Inhibitor, Produced